Journal: Advanced Science

Article Title: IL‐4/STAT6‐signaling Influences Local Inflammation and Regeneration Processes During Acute Pancreatitis and Promotes Fibrosis by a Direct Activation of Pancreatic Fibroblasts During Chronic Pancreatitis

doi: 10.1002/advs.202515585

Figure Lengend Snippet: CD206 M2‐like macrophages differentiate in the absence STAT6 but retain proinflammatory properties (A) Immunofluorescent labeling of CD206, CCR2 and CD3 in CP tissue. (B) Quantification of CD206 + , CCR2 + and CD3 + cells in the pancreas of CP mice ( n = 10). (C) Heat map illustrating different levels of inflammation related proteins between wild type and Stat6‐/‐ mice. The numbers in the boxes represents the change compared with untreated control mice, the amount of all red labeled proteins were significantly changed in Stat6‐/‐ compared to wild type mice after onset of CP ( n = 5). (D) Quantitative gene expression analysis by RT‐qPCR of Arg1 , Fizz , Ym1 and Il10 in CP tissue of wild type and Stat6‐/‐ mice ( n = 5). (E) Immunofluorescent labeling of MMP9 (red) and α‐amylase (green) in CP tissue and quantification of MMP9 + cells in the pancreas of CP mice. (F) Quantitative RT‐PCR of Nos2 , Il1b , Mmp9 and Il6 in CP tissue of wild type and Stat6‐/‐ mice ( n = 10). (G) Measurement of TGF‐β in serum of untreated control mice and CP mice ( n = 9). (H) Quantitative RT‐PCR of Tgfbi and Csf1 in CP tissue of wild type and Stat6‐/‐ mice ( n = 5). Data in B and D‐H are shown as mean +/‐ SEM. Differences were tested for statistical significance by unpaired students t‐test or Mann–Whitney for non‐normally distributed samples for independent samples, significance levels of p < 0.05 are marked by an asterisk.

Article Snippet: Recombinant Mouse IL‐4 Protein (#404‐ML, R&D Systems), Recombinant Mouse IL‐13 Protein (#413‐ML, R&D Systems), Lipopolysaccharide Escherichia coli O26:B6 (L8274, Sigma Aldrich), Recombinant Mouse IL‐6 Protein (406‐ML, R&D systems), Recombinant Mouse TGF‐β Protein (7666‐MB, R&D systems), Recombinant mouse IL‐10 (575804, BioLegend).

Techniques: Labeling, Control, Gene Expression, Quantitative RT-PCR, MANN-WHITNEY

Journal: Nature Immunology

Article Title: Conserved stromal–immune cell circuits secure B cell homeostasis and function

doi: 10.1038/s41590-023-01503-3

Figure Lengend Snippet: a , Heatmap visualizing the average expression of conserved receptor-ligand pairs identified as significant interactions in human SLOs by CellPhoneDB analysis. b , c , Fold changes of SOX9 ( b ) and PI16 ( c ) mRNA levels in bulk cultured primary tonsillar fibroblast of OSA patients stimulated with the indicated recombinant proteins for 48 h and measured by qRT-PCR. d , Fold changes of PI16 mRNA levels after in vitro expansion of sorted PI16 + RCs from human tonsils and following stimulation with TGF-β1 for 48 h. b – d , Box plots with whiskers showing the minimum and maximum values. Horizontal lines indicate the median and boxes represent 0.25−0.75 percentiles ( b , c ) q values are derived from Tukey’s test following Kruskal-Wallis test and using Benjamini, Krieger and Yekutieli correction to control the false discovery rate. ( d ) Two-sided Mann Whitney test was used to test for significant differences. In a , data represent 3,450 CXCL13 + cells and 56,887 immune cells sampled from n = 4 patients for human lymph nodes and n = 4 patients for human palatine tonsils. In b , c , cells from n = 8 patients were cultivated and processed in 2 independent experiments. In d , cells from n = 5 patients were cultivated and processed in 2 independent experiments.

Article Snippet: To evaluate the differentiation and activation potential, 1.5 × 10 4 cells were plated in 24-well plates and stimulated for 48 h with recombinant mouse TGFβ1 (10 ng ml −1 , catalog no. 7666-MB/CF, R&D Systems), recombinant mouse PRGN (10 ng ml −1 , catalog no. 2557-PG, R&D Systems), recombinant mouse VEGF (25 ng ml −1 , catalog no. 493-MV/CF, R&D Systems), recombinant mouse IL-4 (10 ng ml −1 , catalog no. 404-ML/CF, R&D Systems) or recombinant mouse IL-1β (1 ng ml −1 , catalog no. ab259421, Abcam).

Techniques: Expressing, Cell Culture, Recombinant, Quantitative RT-PCR, In Vitro, Derivative Assay, Control, MANN-WHITNEY